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KMID : 0370219950390060585
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Yakhak Hoeji
1995 Volume.39 No. 6 p.585 ~ p.592
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Isolation and Identification of Soil Streptomyces sp. Producing an Immunomodulator That Restores Ultraviolet B Radiation-Induced Suppression of the Immune Response
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모영근/Mo YK
신영근/박동진/김창진/이종길/한성순/Shin YK/Park DJ/Kim CJ/Lee CK/Han SS
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Abstract
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Soil microorganisms producing immunomodulators that can restore ultraviolet B (UVB) radiation-induced suppression of the immune response were screened in vitro. Exposure of freshly isolated murine epidermal cells (EC) to 180 J/m2 of UVB radiation resulted in approximately 90% impairment of accessory cell function, as measured by their ability to support anti-CD3 monoclonal antibody-induced T-cell mitogenesis. When the culture supernatants of 150 actinomycete strains were examined for their capacity to prevent or repair the UVB-induced impairment of accessory cell function, 4 of them were identified to contain immunomodulators that can restore the decreased accessory cell function. The soil isolate that showed the most effective restorative activity, G40025, was selected and further characterized. Addition of 10mcl of the culture supernatant of G4002 grown in G-media to cultures of UVB-irradiated EC right after UVB-irradiation restored the decreased accessory cell function by 58%. The immunomodulator produced by G40025 appeared to be stabled at 100oC for 10 min. Taxonomical studies by cultural, morphological, and physiological characterization showed that the soil isolate, G4002, belongs to the genus Streptomyces.
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